Catecholamine Plasma Membrane Transport Proteins; Neurochemistry; Neuropharmacology; Parkinson Disease
Techniques, methods & infrastructure
- HPLC with electrocehmical detecion for quantitative determination of noradrenaline, dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, serotonin, 5-hydroxyindol acetic acid.
- HPLC with fluorometric detection after precolumn derivatization for quantitative determination of aspartate, glutamate, glutamine, asparagine, glycine, serine, alanine, threonine, taurine, GABA.
- Radioligand binding assays, transport and release (superfusion) assays on synaptosomes, synaptic vesicles, transfected cells.
- Radiochemical assays for glutamic acid decarboxylase, choline acetyl transferase.
- Pifl C, Rajput A, Reither H, Blesa J, Cavada C, Obeso JA, Rajput AH, Hornykiewicz O (2014) Is Parkinson's Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and Nonhuman Primate Striatum J Neurosci, 24: 8210-8218
- Pifl C, Stephen J, Kish SJ, Hornykiewicz O (2012) Thalamic noradrenaline in Parkinson's disease: Deficits suggest role in motor and non-motor symptoms Movement Disorders, 27: 1618-1624
- Peneder TM, Scholze P, Berger ML, Reither H, Heinze G, Bertl J, Bauer J, Richfield EK, Hornykiewicz O, Pifl C (2011) Chronic exposure to manganese decreases striatal dopamine turnover in human alpha-synuclein transgenic mice Neuroscience, 180: 280-292
- Pifl C, Singer EA (1999) Ion dependence of carrier-mediated release in dopamine or norepinephrine transporter-transfected cells questions the hypothesis of faciliated exchange diffusion Mol Pharmacol, 56: 1047-1054
- Sitte HH, Huck S, Reither H, Boehm S, Singer EA, Pifl C (1998) Carrier-mediated release, transport rates and charge transfer induced by amphetamine, tyramine and dopamine in mammalian cells transfected with the human dopamine transporter J Neurochem, 71: 1289-1297