Detail

Keywords

Dental Implants; Inflammation; Periodontal Diseases; Periodontal Ligament; Stem Cell Research; Surface Properties; Titanium; Toll-Like Receptors

Research group(s)

• Competence Center for Periodontal Research
  Head: Oleh Andrukhov
  Members:
  Oleh Andrukhov

Research interests

My main research focus is investigation of cellular and molecular mechanism involved in homeostasis of periodontal tissue in health and disease: regulation of inflammatory response in periodontitis; host interaction with bacteria and bacterial biofilms, dental stem cells and their role in tissue regeneration and immunomodulation; effect of titanium surface roughness on micron- and nanoscale on the cell response and osseointegration. 

Techniques, methods & infrastructure

In vitro cell culture of different human primary cells (e.g. monocytes, T-cells, endothelial cells, epithelial cells, dental stem cells, osteoblasts).  A broad spectrum of biochemical, cell biological, and imaging techniques are used. Time lapse microsopy: observation and quantification of cell behaviour for prolonged time (up to 2 weeks).

Grants

• Vitamin D and immunomodulation by mesenchymal stem cells (2016)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator

Selected publications

1. Behm, C. et al. (2024) ‘Heterogeneity in Dental Tissue–Derived MSCs Revealed by Single-Cell RNA-seq’, Journal of Dental Research, 103(11), pp. 1141–1152. Available at: https://doi.org/10.1177/00220345241271997.
2. Behm, C. et al. (2024) ‘Paracrine- and cell-contact-mediated immunomodulatory effects of human periodontal ligament-derived mesenchymal stromal cells on CD4+ T lymphocytes’, Stem Cell Research & Therapy, 15(1). Available at: https://doi.org/10.1186/s13287-024-03759-4.
3. Andrukhov, O. et al. (2019) ‘Immunomodulatory properties of dental tissue-derived mesenchymal stem cells: Implication in disease and tissue regeneration’, World Journal of Stem Cells, 11(9), pp. 604–617. Available at: https://doi.org/10.4252/wjsc.v11.i9.604.
4. Blufstein, A. et al. (2024) ‘Salivary microbiome and MRP‐8/14 levels in children with gingivitis, healthy children, and their mothers’, Journal of Periodontology, 95(11), pp. 1035–1047. Available at: https://doi.org/10.1002/jper.23-0632.
5. Andrukhov, O. et al. (2020) ‘Effect of implant surface material and roughness to the susceptibility of primary gingival fibroblasts to inflammatory stimuli’, Dental Materials, 36(6), pp. e194–e205. Available at: https://doi.org/10.1016/j.dental.2020.04.003.