Ao.Prof. Dr. Christian Pifl
Center for Brain Research (Division of Molecular Neurosciences)
Position: Associate Professor
christian.pifl@meduniwien.ac.at
Keywords
Catecholamine Plasma Membrane Transport Proteins; Neurochemistry; Neuropharmacology; Parkinson Disease
Techniques, methods & infrastructure
- HPLC with electrocehmical detecion for quantitative determination of noradrenaline, dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, serotonin, 5-hydroxyindol acetic acid.
- HPLC with fluorometric detection after precolumn derivatization for quantitative determination of aspartate, glutamate, glutamine, asparagine, glycine, serine, alanine, threonine, taurine, GABA.
- Radioligand binding assays, transport and release (superfusion) assays on synaptosomes, synaptic vesicles, transfected cells.
- Radiochemical assays for glutamic acid decarboxylase, choline acetyl transferase.
Selected publications
- Bhat, S. et al. (2023) ‘A mechanism of uncompetitive inhibition of the serotonin transporter’, eLife, 12. Available at: http://dx.doi.org/10.7554/elife.82641.
- Hörtnagl, H. et al. (2019) ‘Distinct gradients of various neurotransmitter markers in caudate nucleus and putamen of the human brain’, Journal of Neurochemistry, 152(6), pp. 650–662. Available at: http://dx.doi.org/10.1111/jnc.14897.
- Pifl, C. et al. (2014) ‘Is Parkinson’s Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and Nonhuman Primate Striatum’, Journal of Neuroscience, 34(24), pp. 8210–8218. Available at: http://dx.doi.org/10.1523/jneurosci.5456-13.2014.
- Pifl, C., Reither, H. and Hornykiewicz, O. (2015) ‘The profile of mephedrone on human monoamine transporters differs from 3,4-methylenedioxymethamphetamine primarily by lower potency at the vesicular monoamine transporter’, European Journal of Pharmacology, 755, pp. 119–126. Available at: http://dx.doi.org/10.1016/j.ejphar.2015.03.004.
- Sitte HH, Huck S, Reither H, Boehm S, Singer EA, Pifl C (1998) Carrier-mediated release, transport rates and charge transfer induced by amphetamine, tyramine and dopamine in mammalian cells transfected with the human dopamine transporter J Neurochem, 71: 1289-1297