Detail

Keywords

Catecholamine Plasma Membrane Transport Proteins; Neurochemistry; Neuropharmacology; Parkinson Disease

Techniques, methods & infrastructure

• HPLC with electrocehmical detecion for quantitative determination of noradrenaline, dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, serotonin, 5-hydroxyindol acetic acid.

• HPLC with fluorometric detection after precolumn derivatization for quantitative determination of aspartate, glutamate, glutamine, asparagine, glycine, serine, alanine, threonine, taurine, GABA.

• Radioligand binding assays, transport and release (superfusion) assays on synaptosomes, synaptic vesicles, transfected cells.

• Radiochemical assays for glutamic acid decarboxylase, choline acetyl transferase.

Selected publications

1. Pifl, C. et al. (2023) ‘Dopamine and vesicular monoamine transport loss supports incidental Lewy body disease as preclinical idiopathic Parkinson’, npj Parkinson’s Disease, 9(1). Available at: https://doi.org/10.1038/s41531-023-00514-z.
2. Bhat, S. et al. (2023) ‘A mechanism of uncompetitive inhibition of the serotonin transporter’, eLife, 12. Available at: https://doi.org/10.7554/elife.82641.
3. Hörtnagl, H. et al. (2019) ‘Distinct gradients of various neurotransmitter markers in caudate nucleus and putamen of the human brain’, Journal of Neurochemistry, 152(6), pp. 650–662. Available at: https://doi.org/10.1111/jnc.14897.
4. Pifl, C. et al. (2014) ‘Is Parkinson’s Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and Nonhuman Primate Striatum’, Journal of Neuroscience, 34(24), pp. 8210–8218. Available at: https://doi.org/10.1523/jneurosci.5456-13.2014.
5. Sitte, H.H. et al. (1998) ‘Carrier‐Mediated Release, Transport Rates, and Charge Transfer Induced by Amphetamine, Tyramine, and Dopamine in Mammalian Cells Transfected with the Human Dopamine Transporter’, Journal of Neurochemistry, 71(3), pp. 1289–1297. Available at: https://doi.org/10.1046/j.1471-4159.1998.71031289.x.