Allergy and Immunology; Colitis; High-Throughput Nucleotide Sequencing; Innate Immunity; MicroRNAs; RNA Editing
Today, over 100 different chemical modifications of RNA have been described. The complexity and impact of these modifications in thousands of RNAs is adding another layer in regulating gene expression and sparked the field of epitranscriptomics with impact on biology and medicine. Within an interdisciplinary researcher consortium we tackle questions concerning epitranscriptomic A to I RNA modification and its implications in inflammatory bowel diseases, the crosstalk of the host & the gut microbiota and the mechanisms ensuring immune homeostasis, utilizing mouse models and modern sequencing and CRISPR/Cas9 technologies.
- Mannion, N.M. et al., 2014. The RNA-Editing Enzyme ADAR1 Controls Innate Immune Responses to RNA. Cell Reports, 9(4), pp.1482–1494. Available at: http://dx.doi.org/10.1016/j.celrep.2014.10.041.
- Vesely, C. et al., 2014. ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain. Nucleic Acids Research, 42(19), pp.12155–12168. Available at: http://dx.doi.org/10.1093/nar/gku844.
- Vesely, C. et al., 2012. Adenosine deaminases that act on RNA induce reproducible changes in abundance and sequence of embryonic miRNAs. Genome Research, 22(8), pp.1468–1476. Available at: http://dx.doi.org/10.1101/gr.133025.111.