Center for Physiology and Pharmacology (Division of Neurophysiology and Neuropharmacology)
Position: Research Associate (Postdoc)
ORCID: 0000-0002-0482-9401
matej.hotka@meduniwien.ac.at
Keywords
Calcium Channels, L-Type; Epilepsy; Metabolism; Mitochondria; Neurons
Research interests
My main research focus is to understand the proces of epileptogenesis and the role that neuronal metabolism plays in it. More specifically, we study how neuronal firing of various intensities affects mitochondrial ATP production and glycolysis.
Techniques, methods & infrastructure
We use primary cultures of mouse and rat hippocampal neurons. For the investigations of neuronal metabolism and calcium signaling we use neurons transiently expressing/stained with fluorescent-based indicators and imaging by laser-scanning confocal microscopy. Neuronal activity is assesed by minimally invasive perforated patch recordings using patch-clamp electrophysiology.
Grants
- Paroxysmal depolarization protects neurons via mitochondria (2024)
Source of Funding: FWF (Austrian Science Fund), Principal Investigator Projects
Principal Investigator - Regulation of mitochondrial ATP synthesis by neuronal Cav1 (2020)
Source of Funding: FWF (Austrian Science Fund), Standalone project
Principal Investigator
Selected publications
- Dhoundiyal, A. et al. (2022) ‘Glycerol-3-Phosphate Shuttle Is a Backup System Securing Metabolic Flexibility in Neurons’, The Journal of Neuroscience, 42(39), pp. 7339–7354. Available at: https://doi.org/10.1523/jneurosci.0193-22.2022.
- Hotka, M. and Kubista, H. (2019) ‘The paroxysmal depolarization shift in epilepsy research’, The International Journal of Biochemistry & Cell Biology, 107, pp. 77–81. Available at: http://dx.doi.org/10.1016/j.biocel.2018.12.006.
- Kubista, H., Boehm, S. and Hotka, M. (2019) ‘The Paroxysmal Depolarization Shift: Reconsidering Its Role in Epilepsy, Epileptogenesis and Beyond’, International Journal of Molecular Sciences, 20(3), p. 577. Available at: https://doi.org/10.3390/ijms20030577.
- Hotka, M. and Kubista, H. (2019) ‘The paroxysmal depolarization shift in epilepsy research’, The International Journal of Biochemistry & Cell Biology, 107, pp. 77–81. Available at: https://doi.org/10.1016/j.biocel.2018.12.006.
- Burtscher, V. et al. (2018) ‘A label-free approach to detect ligand binding to cell surface proteins in real time’, eLife, 7. Available at: https://doi.org/10.7554/elife.34944.