Cell Biology; Cell Hypoxia; Dental Pulp; Endodontics; Fibroblasts; Guided Tissue Regeneration, Periodontal; Hypoxia-Inducible Factor 1; Osteoblasts; Osteoclasts; Tissue Engineering; Tissue Scaffolds
Inspired by the clinic the research of our team focuses on regeneration of oral tissues including dental pulp, periodontal ligament, and bone through tissue engineering and cell-based therapies. To accomplish this we apply molecular and cellular techniques and evaluate the mechanisms underlying regeneration and the response to biologicals, pharmaceuticals, and biomaterials. Preclinical and clinical approaches help us to reveal the therapeutics potential of new strategies. Furthermore, we apply the knowledge of cell biology to develop novel in vitro models, which closer mimic the in vivo situation to evaluate treatment strategies and thereby reduce the number of animal experiments.
Other interests: Supervision, mentoring and coaching
Techniques, methods & infrastructure
Cell culture (e.g.: fibroblasts from the gingiva and the periodontal ligament, dental pulp-derived cells, osteogenic cells, endothelial cells, and cell lines), 3D in vitro models, spheroid cultures, tooth slice organ cultures, osteoblastogenesis assays, osteoclastogenesis assays, qPCR, ELISA, western blot, zymography, enzyme activity assays, immunohistochemistry, evaluation of the release kinetic of biomaterials.
- Müller, H.-D. et al., 2015. Effects of Prolyl Hydroxylase Inhibitor L-mimosine on Dental Pulp in the Presence of Advanced Glycation End Products. Journal of Endodontics, 41(11), pp.1852-1861. Available at: http://dx.doi.org/10.1016/j.joen.2015.08.002.
- Agis, H. et al., 2014. Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound Repair C. Egles, ed. PLoS ONE, 9(11), p.e112680. Available at: http://dx.doi.org/10.1371/journal.pone.0112680.
- Agis, H. et al., 2013. Platelets increase while serum reduces the differentiation and activity of osteoclasts in vitro. J. Orthop. Res., 31(10), pp.1561-1569. Available at: http://dx.doi.org/10.1002/jor.22386.
- Agis, H., Watzek, G. & Gruber, R., 2011. Prolyl hydroxylase inhibitors increase the production of vascular endothelial growth factor by periodontal fibroblasts. Journal of Periodontal Research, 47(2), pp.165-173. Available at: http://dx.doi.org/10.1111/j.1600-0765.2011.01415.x.
- Agis, H. et al., 2009. Is Zoledronate Toxic to Human Periodontal Fibroblasts? Journal of Dental Research, 89(1), pp.40-45. Available at: http://dx.doi.org/10.1177/0022034509354298.