Adaptive Immunity; Bacterial Infections; Immunity, Innate; Intercellular Signaling Peptides and Proteins; Intracellular Signaling Peptides and Proteins; Macrophages
- Molecular Immunology Unit
I am interested in the structure and function of surface receptors on T cells and accessory cells (macrophages, dendritic cells) to identify novel targets for influencing abnormal and unwanted immune reactions in immunological disorders and diseases. Internationally well recognized are the investigations, which contribute to the understanding of how glycosylphosphatidylinositol (GPI)-anchored receptor proteins transduce signals across the plasma membrane. These studies were fundamental for the identification and characterization of special membrane microdomains, called lipid rafts, which are more and more characterized to control initiation of signal transduction across the plasma membrane of cells. Currently, I am focusing on three main projects: 1) on the signal transduction mechanism of the T cell antigen receptor (TCR), in particular how Lck, the key signalling kinase, is regulated as well as how positive and negative regulating transmembrane accessory molecules control TCR signalling, 2) to characterize human pathological macrophages in autoimmune diseases and to target them for therapeutic corrections by special nanodevices, 3) to develop novel detection platforms for the diagnosis of bacterial infections.
Techniques, methods & infrastructure
Cell culture, multi-parameter flow cytometry, molecular cloning, shRNA silencing, various immunological techniques (isolation, cultivation and stimulation of immune cells, ELISA, immunofluorescence, immunobiochemistry including immunoprecipitation and multiplex immunoblotting, etc), chromatography, advanced imaging (single molecule analysis). Top-level infrastructure including tissue culture, HPLC, FPLC, various immunodetection systems including ELISAs and Luminex, super-resolution fluorescence microscope for live cell imaging and two advanced flow cytometers able to analyse up to 18 cellular parameters, that are crucial for multicolour and functional analyses of immune cells.
- FOLSMART - Folate-Target Nanodevies To Activated Macrophages For Rheumatoid Arthritis (project partner) (2015)
Source of Funding: EU, H2020-NMP-PILOTS-2015
- Cell Communication in Health and Disease, CCHD (Member) (2009)
Source of Funding: FWF (Austrian Science Fund), Doctoral Program
- NANOFOL - Folate-based nanoblodevices for integrated diagnosis/therapy targeting chronic inflammatory diseases (project partner) (2009)
Source of Funding: EU, FP7-NMP-2008-LARGE-2
- Schatzlmaier, P. et al., 2015. Rapid multiplex analysis of lipid raft components with single-cell resolution. Science Signaling, 8(395), pp.rs11-rs11. Available at: http://dx.doi.org/10.1126/scisignal.aac5584.
- Leksa, V. et al., 2011. Soluble M6P/IGF2R Released by TACE Controls Angiogenesis via Blocking Plasminogen Activation. Circulation Research, 108(6), pp.676-685. Available at: http://dx.doi.org/10.1161/CIRCRESAHA.110.234732.
- Schwarzenbacher, M. et al., 2008. Micropatterning for quantitative analysis of protein-protein interactions in living cells. Nat Meth, 5(12), pp.1053-1060. Available at: http://dx.doi.org/10.1038/nmeth.1268.
- Bohuslav, J., 1995. Urokinase plasminogen activator receptor, beta 2-integrins, and Src- kinases within a single receptor complex of human monocytes. Journal of Experimental Medicine, 181(4), pp.1381-1390. Available at: http://dx.doi.org/10.1084/jem.181.4.1381.
- Stefanova, I. et al., 1991. GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science, 254(5034), pp.1016-1019. Available at: http://dx.doi.org/10.1126/science.1719635.