Allergy and Immunology; Humans; Inflammation; Molecular Biology; Skin; Wound Healing
- Adelheid Elbe-Bürger
Topical antiseptics are less potent to induce resistance using their unspecific mode of action and the high concentrations in which they can be used comparing to the use of antibiotics. Contrasting with other antiseptic substances, octenidine dihydrochloride (OCT) shows high efficiency already within a short application time at low concentrations. Recent studies in humans demonstrated that OCT does not delay the wound epithelization and significantly lowers bacterial colonization on skin wounds leading to a greater reduction in wound size and higher healing rates of skin graft wounds in burn patients. Results from animal and clinical observations imply that OCT may have a positive influence on wound healing. Yet, the exact influence of OCT on human skin cells has not been fully investigated.
We use a human skin explant model to study the OCT effects on injuries caused by tape-stripping, insisional wounding method, as well as by partial thickness wounding to investigate the influence on wound clousure. The primary purpose is to study the influence of OCT on immune and non-immune cells and the quantification of multiple inflammatory mediators associated with wound healing in culture supernatants, such as cytokines, chemokines and growth factors, as well as on the skin reepithelialization.
Techniques, methods & infrastructure
Human ex vivo (wounded) skin explant cultures