Allergy and Immunology
- Allergy Research
Research Area: focuses on the elucidation of the immune mechanisms operative in the induction, maintenance and treatment of allergic disorders and the development of novel concepts for efficient and safe allergen-specific immunotherapy.
We are interested in elucidating the immunological mechanisms underlying the induction, maintenance and effective treatment of IgE-mediated allergy. Moreover, we characterize allergens and develop novel concepts for allergen-specific immunotherapy.
Techniques, methods & infrastructure
Our expertise includes a broad spectrum of state-of-the-art-methodologies in cellular and molecular biology and immunology, e.g. human cell culture, magnetic cell sorting, FACS, intracellular cytokine detection, real-time PCR, live cell imaging, T cell cloning and T cell epitope mapping systems, ELISA, and immunoblotting.
We are specialized in the isolation and characterization of primary immune cells (T, B, DC, Monocytes), and allergen-specific T cell lines and clones. In addition, we perform various read-out systems for allergic responses, e.g. basophil activation assays, mediator-release experiments, facilitated allergen-binding assays, and study antigen-processing by means of antigen-uptake and degradation assays, endolysosomal proteolysis, and presentation to specific T cells.
- Kitzmüller, C. et al., 2015. Correlation of sensitizing capacity and T-cell recognition within the Bet v 1 family. Journal of Allergy and Clinical Immunology, 136(1), pp.151-158. Available at: http://dx.doi.org/10.1016/j.jaci.2014.12.1928.
- Kinaciyan, T. et al., 2015. Recombinant Mal d 1 facilitates sublingual challenge tests of birch pollen-allergic patients with apple allergy. Allergy, p.n/a-n/a. Available at: http://dx.doi.org/10.1111/all.12781.
- Deifl, S. et al., 2014. Differential activation of dendritic cells by toll-like receptors causes diverse differentiation of naive CD4 + T cells from allergic patients . Allergy, 69(12), pp.1602-1609. Available at: http://dx.doi.org/10.1111/all.12501.
- Subbarayal, B. et al., 2013. Kinetics, cross-reactivity, and specificity of Bet v 1-specific IgG4 antibodies induced by immunotherapy with birch pollen. Allergy, 68(11), pp.1377-1386. Available at: http://dx.doi.org/10.1111/all.12236.