Skip to main content

Detail

Barbara Bohle
Barbara BohleHead of the Department of Pathophysiology and Allergy Research

Center for Pathophysiology, Infectiology and Immunology (Institute of Pathophysiology and Allergy Research)
Position: Professor

T +43 1 40400 51140
barbara.bohle@meduniwien.ac.at

Further Information

Keywords

Allergy and Immunology

Research group(s)

  • Allergy Research

Research interests

We are interested in elucidating the immunological mechanisms underlying the induction, maintenance and effective treatment of IgE-mediated allergy. Moreover, we characterize allergens and develop novel concepts for allergen-specific immunotherapy. 

Techniques, methods & infrastructure

Our expertise includes a broad spectrum of state-of-the-art-methodologies in cellular and molecular biology and immunology, e.g. human cell culture, magnetic cell sorting, FACS, intracellular cytokine detection, real-time PCR, live cell imaging, T cell cloning and T cell epitope mapping systems, ELISA, and immunoblotting.

We are specialized in the isolation and characterization of primary immune cells (T, B, DC, Monocytes), and allergen-specific T cell lines and clones. In addition, we perform various read-out systems for allergic responses, e.g. basophil activation assays, mediator-release experiments, facilitated allergen-binding assays, and study antigen-processing by means of antigen-uptake and degradation assays, endolysosomal proteolysis, and presentation to specific T cells.

Selected publications

  1. Kitzmüller, C. et al., 2015. Correlation of sensitizing capacity and T-cell recognition within the Bet v 1 family. Journal of Allergy and Clinical Immunology, 136(1), pp.151-158. Available at: http://dx.doi.org/10.1016/j.jaci.2014.12.1928.
  2. Kinaciyan, T. et al., 2015. Recombinant Mal d 1 facilitates sublingual challenge tests of birch pollen-allergic patients with apple allergy. Allergy, p.n/a-n/a. Available at: http://dx.doi.org/10.1111/all.12781.
  3. Deifl, S. et al., 2014. Differential activation of dendritic cells by toll-like receptors causes diverse differentiation of naive CD4 + T cells from allergic patients . Allergy, 69(12), pp.1602-1609. Available at: http://dx.doi.org/10.1111/all.12501.
  4. Subbarayal, B. et al., 2013. Kinetics, cross-reactivity, and specificity of Bet v 1-specific IgG4 antibodies induced by immunotherapy with birch pollen. Allergy, 68(11), pp.1377-1386. Available at: http://dx.doi.org/10.1111/all.12236.