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Johannes Schmid
Assoc. Prof., PhD Johannes SchmidSpeaker of the Special Research Programme SFB-F54 "Inflammation and Thrombosis"

Center for Physiology and Pharmacology (Institute of Vascular Biology and Thrombosis Research)
Position: Associate Professor

T +43 1 40160 31155
johannes.schmid@meduniwien.ac.at

Further Information

Keywords

DNA; Fluorescence; Imaging, Three-Dimensional; Inflammation; Microscopy; NF-kappa B; Oncology; Protein Interaction Maps; Signal Transduction; Thrombosis; Transcription Factors

Research group(s)

  • Johannes Schmid

Research interests

Current research interests focus on inflammation, thrombosis and cancer. Signal transduction pathways of inflammation are studied with special focus on the NF-kappa B signaling, as well as interconnections with other signaling processes in vascular biology and cancer .   Signal transduction networks are studied with a variety of experimental systems, such as cell culture of primary and transformed cells or transgene mouse models. Transfection and viral transduction strategies are applied to achieve either ectopic expression or gene suppression of effector molecules followed by analyzing a variety of biological readouts such as cell proliferation, apoptosis or activation of cells.

Techniques, methods & infrastructure

  • epifluorescence microscopy (of life cells)
  • laser scanning microscopy including FLIP and FRAP microscopy
  • FRET (Fluorescence Resonance Energy Transfer) measurements and microscopy
  • DNA-protein binding assays (EMSA's and non-radioactive alternatives)
  • protein-protein interaction assays (co-immunoprecipitation, FRET, yeast 2-hybrid, mammalian 2-hybrid etc.)
  • reporter gene assays
  • flow cytometry and cell sorting
  • tissue cytometry
  • histology
  • kinase assays
  • realtime PCRs
  • Northern Blots
  • molecular biology and cloning
  • biochemistry methods
  • cell biology methods
  • transfections
  • RNA interference
  • radioactive labeling
  • ultra-centrifugation methods (subcellular fractionation)
  • fluorometry
  • chromatography
  • in situ hybridizations
  • cell cycle profiling
  • apoptosis assays
  • proliferation assays
  • in vitro fusion of cellular compartments
  • concepts of genome editing (CRISPR/Cas9)

Grants

  • Dynamics of molecular interactions in live cells (2015)
    Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
    Principal Investigator
  • Cellular Mediators Linking Inflammation and Thrombosis (2013)
    Source of Funding: FWF (Austrian Science Fund), Special Research Programmes (SFB54)
    Coordinator of the collaborative project
  • Transcription Factors in Inflammation and Cancer (2011)
    Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
    Principal Investigator

Selected publications

  1. Hoesel, B. & Schmid, J.A., 2013. The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer, 12(1), p.86. Available at: http://dx.doi.org/10.1186/1476-4598-12-86.
  2. Neoplasia. 2011 Aug;13(8):692-703. Persistent inflammation leads to proliferative neoplasia and loss of smooth muscle cells in a prostate tumor model. Birbach A, Eisenbarth D, Kozakowski N, Ladenhauf E, Schmidt-Supprian M, Schmid JA.
  3. Sughra, K. et al., 2010. Interaction of the TNFR-Receptor Associated Factor TRAF1 with I-Kappa B Kinase-2 and TRAF2 Indicates a Regulatory Function for NF-Kappa B Signaling S. Agarwal, ed. PLoS ONE, 5(9), p.e12683. Available at: http://dx.doi.org/10.1371/journal.pone.0012683.
  4. Schmid, J.A. & Birbach, A., 2008. IκB kinase β (IKKβ/IKK2/IKBKB) A key molecule in signaling to the transcription factor NF-κB. Cytokine & Growth Factor Reviews, 19(2), pp.157-165. Available at: http://dx.doi.org/10.1016/j.cytogfr.2008.01.006.
  5. Orel, L. et al., 2009. Crosstalk between the NF-κB activating IKK-complex and the CSN signalosome. Journal of Cellular and Molecular Medicine, 14(6b), pp.1555-1568. Available at: http://dx.doi.org/10.1111/j.1582-4934.2009.00866.x.