Acute Kidney Injury; Gene Knockdown Techniques; Graft Rejection; Kidney Transplantation; Kidney Tubular Necrosis, Acute; Loss of Heterozygosity; Microarray Analysis; MicroRNAs; RNA, Small Interfering
- Rainer Oberbauer
I am interested in the understanding of the molecular regulatory mechanisms and the immunological modulation in several kidney diseases after kidney allograft transplantation. In addition the clonal B- and T-cell expansion, the role of loss of function mutations and knock down mechanisms with small non coding RNAs in allograft rejection are our major research interests.
In the recent past, the focus of my research was the identification of non-invasive biomarkers in bodily fluids of patients with allograft rejection. Therefore I performed whole transcript analysis from kidney biopsy samples and I am now interested in the involved diseases of the identified significantly regulated profile.
Techniques, methods & infrastructure
For gene expression analysis of mRNA and microRNA our research group uses renal needle biopsies from patients with allograft rejection or tissue from mice/rats/pigs after administration of antisense nucleotides. MicroRNA and mRNA profiles are determined using microarray technology and qRT-PCR (Wilflingseder et al. 2014). Further Next Generation Sequencing was used to analyze the clonal B- and T-cell repertoire in kidney diseases (Weinberger et al., 2015).
Protein level detection is done via Western blotting, ELISA or bead based methods (e.g. Luminex technology).
Our research group has access to molecular biology labs for qRT-PCR, Next Generation Sequencing and microarray analysis as well as a fully equipped cell culture lab, a Laser Capture Microdissection unit and protein technologies including a Luminex 200.
- Rudnicki, M. et al., 2015. Renal microRNA- and RNA-profiles in progressive chronic kidney disease. European Journal of Clinical Investigation, p.n/a-n/a. Available at: http://dx.doi.org/10.1111/eci.12585.
- Weinberger, J. et al., 2015. Immune Repertoire Profiling Reveals that Clonally Expanded B and T Cells Infiltrating Diseased Human Kidneys Can Also Be Tracked in Blood G. Lythe, ed. PLoS ONE, 10(11), p.e0143125. Available at: http://dx.doi.org/10.1371/journal.pone.0143125.
- Wilflingseder, J. et al., 2014. MicroRNAs in kidney transplantation. Nephrology Dialysis Transplantation, 30(6), pp.910-917. Available at: http://dx.doi.org/10.1093/ndt/gfu280.
- Wilflingseder, J. et al., 2014. Molecular Pathogenesis of Post-Transplant Acute Kidney Injury: Assessment of Whole-Genome mRNA and MiRNA Profiles G. Camussi, ed. PLoS ONE, 9(8), p.e104164. Available at: http://dx.doi.org/10.1371/journal.pone.0104164.
- Rudnicki, M. et al., 2012. Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease J.-C. Dussaule, ed. PLoS ONE, 7(9), p.e44891. Available at: http://dx.doi.org/10.1371/journal.pone.0044891.