Detail

Keywords

Cytoprotection; Heat-Shock Response; Peritoneal Dialysis; Translational Medical Research; Ultrafiltration

Research interests

My laboratory focuses on cytotoxic damage in the model of peritoneal dialysis (PD). We describe and manipulate the cellular stress response in different in-vitro and in-vivo models of experimental PD, allowing improved cellular survival. Using systems-biology approaches we showed that stress responses in PD were not adequate for sufficient cytoprotection. In cooperation with the MedUni Vienna spin-off Zytoprotec, novel therapeutic interventions are developed in order to re-establish adequate cellular repair mechanism. Parallel to basic research we perform clinical research on the optimization of therapy for pediatric kidney transplantations and dialysis.Translational and clinical-epidemiological studies led to the establishment of an interdisciplinary working group that brought improvements in the quality of our daily clinical care. All junior researchers are participating in the N790 PhD program POeT (Program for Organfailure-, replacement and Transplantation)

Techniques, methods & infrastructure

Different models of experimental PD: biochemical analysis including FACS, Western blot, Immunofluorescence, proteomics, transcriptomics, metabolomics and clinical-epidemiological tools in cooperation with international registries (ERA-EDTA).

Selected publications

1. Eibensteiner F., Ritschl V., Ariceta G., Jankauskiene A., Klaus G., Paglialonga F., Edefonti A., Ranchin B., Schmitt CP., Shroff R., Stefanidis CJ., Walle JV., Verrina E., Vondrak K., Zurowska A., Stamm T., Aufricht C. Rapid response in the COVID-19 pandemic: a Delphi study from the European Pediatric Dialysis Working Group. Pediatr Nephrol. 2020 Sep;35(9):1669-1678. doi: 10.1007/s00467-020-04584-6. Epub 2020 May 17. Pubmed-Link: https://pubmed.ncbi.nlm.nih.gov/32418146/
2. Vychytil A., Herzog R., Probst P., Ribitsch W., Lhotta K., Machold-Fabrizii V., Wiesholzer M., Kaufmann M., Salmhofer H., Windpessl M., Rosenkranz AR., Oberbauer R., König F., Kratochwill K., Aufricht C. A randomized controlled trial of alanyl-glutamine supplementation in peritoneal dialysis fluid to assess impact on biomarkers of peritoneal health. Kidney Int. 2018 Dec;94(6):1227-1237. doi: 10.1016/j.kint.2018.08.031. Epub 2018 Oct 22. Pubmed-Link: https://www.ncbi.nlm.nih.gov/pubmed/30360960
3. Herzog R., Boehm M., Unterwurzacher M., Wagner A., Parapatics K., Májek P., Lichtenauer A., Bennett KL., Alper SL., Vychytil A., Aufricht C., Kratochwill K. Effects of Alanyl-Glutamine Treatment on the Peritoneal Dialysis Effluent Proteome Reveal Pathomechanism-Associated Molecular Signatures. Mol Cell Proteomics. 2018 Mar;17(3):516-532. doi: 10.1074/mcp.RA117.000186. Epub 2017 Dec 4. Pubmed-Link: https://www.ncbi.nlm.nih.gov/pubmed/29208752
4. Aufricht C., Beelen R., Eberl M., Fischbach M., Fraser D., Jörres A., Kratochwill K., LópezCabrera M., Rutherford P., Schmitt CP., Topley N., Witowski J. Biomarker research to improve clinical outcomes of peritoneal dialysis: consensus of the European Training and Research in Peritoneal Dialysis (EuTRiPD) network. Kidney Int. 2017 Oct;92(4):824-835. doi: 10.1016/j.kint.2017.02.037. Epub 2017 Aug 8. Pubmed-Link: https://www.ncbi.nlm.nih.gov/pubmed/28797473
5. Bidmon-Fliegenschnee B., Lederhuber H., Csaicsich D., Pichler J., Herzog R., Memaran-Dadgar N., Huber W.D., Aufricht C., Kratochwill K. Overexpression of Hsp70 confers cytoprotection during gliadin exposure in Caco-2 cells. Pediatr Res. 2015 Oct;78(4):358-64. doi: 10.1038/pr.2015.112. Epub 2015 Jun 18. Pubmed-Link: http://www.ncbi.nlm.nih.gov/pubmed/26086640