Hemodynamics; Neuroimaging; Ophthalmology; Tomography, Optical Coherence
My main research interests are focused on the development and application of optical imaging techniques, specifically optical coherence tomography (OCT), to study biomedical systems for preclinical or clinical research. In particular, I am interested in the role of microvascular hemodynamics in the progression of various brain and eye diseases.
Techniques, methods & infrastructure
I primarily use Optical Coherence Tomography (OCT) for brain and eye imaging in small animal (mouse and rat) models. In addition to traditional OCT methods, I have also developed new methods based on spectral and exogenous contrast.
- Merkle, C.W. et al., 2021. High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice. Biomedical Optics Express, 12(4), p.1774. Available at: http://dx.doi.org/10.1364/BOE.415227.
- Merkle, C.W. et al., 2020. Indocyanine green provides absorption and spectral contrast for optical coherence tomography at 840 nm in vivo. Optics Letters, 45(8), p.2359. Available at: http://dx.doi.org/10.1364/OL.380051.
- Merkle, C.W. et al., 2019. Dynamic Contrast Optical Coherence Tomography reveals laminar microvascular hemodynamics in the mouse neocortex in vivo. NeuroImage, 202, p.116067. Available at: http://dx.doi.org/10.1016/j.neuroimage.2019.116067.
- Merkle, C.W. et al., 2018. Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo. Optics Letters, 43(2), p.198. Available at: http://dx.doi.org/10.1364/OL.43.000198.
- Merkle, C.W. & Srinivasan, V.J., 2016. Laminar microvascular transit time distribution in the mouse somatosensory cortex revealed by Dynamic Contrast Optical Coherence Tomography. NeuroImage, 125, pp.350–362. Available at: http://dx.doi.org/10.1016/j.neuroimage.2015.10.017.