Detail

Keywords

Bleeding Time; Blood Coagulation Factors; Body Fluids; Cancer-associated thrombosis ; Hemophilia A; Hemophilia B

Research group(s)

• Thrombosis and Haemostasis Research Group
  Head: Cihan Ay
  Research Area: Our research focuses on thrombosis and haemostasis, with a particular interest in improving the understanding of risk, clinical course and mechanisms of venous thrombosis and bleeding disorders such as haemophilia. The Thrombosis & Haemostasis Research Group is part of the Department of Medicine I, Clinical Division of Haemotology and Haemostaseology, and jointly affiliated with Comprehensive Cancer Center, Cardiovascular Cluster and the Comprehensive Center for Cardiovascular Medicine.
  Members:
  Cihan Ay
  Dino Mehic
  Oliver Königsbrügge
  Johannes Thaler

Research interests

My main scientific focus is on the investigation of coagulant extracellular vesicles, tissue factor, and activated factor VII in clinical samples. Another focus is on coagulation assay development and the investigation of potential biomarkers for the prediction of venous thromboembolism in cancer patients.

Techniques, methods & infrastructure

My lab is located in the Anna Spiegel Forschungsgebäude of the Medical University of Vienna. We developed and established dedicated assays (such as an in-house factor Xa generation assay for the quantification of extrinsic tenase complexes, a modified thrombin generation assay, modified whole blood and plasma clotting assays) for the quantification of coagulant extracellular vesicles in clinical samples (e.g. amniotic fluid, ascites, blood, plasma, pleural effusions, human milk, saliva, and urine). We apply state of the art methods for the isolation of extracellular vesicles such as size exclusion chromatography and differential high speed centrifugation and use monoclonal antibodies for the specific blockade of coagulation pathways in buffer, normal plasma or different factor deficient plasmas.