Detail

Keywords

Genomics; Proteomics; Systems Biology; Virology

Research group(s)

• Hein Lab
  Head: Marco Hein
  Members:
  Marco Hein

Research interests

My fundamental research question is understanding the principles of the organization of life at the molecular level. My goal is to design systems-level experiments that yield systems-level insight. Specifically, my team studies the interactions of viruses with their hosts, focusing on what happens in a host cell once it is infected with a virus, or in an antiviral state.

Techniques, methods & infrastructure

We use modern methods of mass spectrometry-based proteomics, CRISPR/Cas9-based functional genomics, single-cell transcriptomics, computational biology, and data visualization.

Selected publications

1. Hein, M.Y. et al. (2024) ‘Global organelle profiling reveals subcellular localization and remodeling at proteome scale’, Cell. Available at: https://doi.org/10.1016/j.cell.2024.11.028.
2. Sunshine, S. et al. (2023) ‘Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq’, Nature Communications, 14(1). Available at: https://doi.org/10.1038/s41467-023-41788-4.
3. Hein, M.Y. and Weissman, J.S. (2021) ‘Functional single-cell genomics of human cytomegalovirus infection’, Nature Biotechnology, 40(3), pp. 391–401. Available at: https://doi.org/10.1038/s41587-021-01059-3.
4. Cho, N.H. et al. (2022) ‘OpenCell: Endogenous tagging for the cartography of human cellular organization’, Science, 375(6585). Available at: https://doi.org/10.1126/science.abi6983.
5. Hein, M.Y. et al. (2015) ‘A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances’, Cell, 163(3), pp. 712–723. Available at: https://doi.org/10.1016/j.cell.2015.09.053.