Genomics; Proteomics; Systems Biology; Virology
My fundamental research question is understanding the principles of the organization of life at the molecular level. My goal is to design systems-level experiments that yield systems-level insight. Specifically, my team studies the interactions of viruses with their hosts, focusing on what happens in a host cell once it is infected with a virus, or in an antiviral state.
Techniques, methods & infrastructure
We use modern methods of mass spectrometry-based proteomics, CRISPR/Cas9-based functional genomics, single-cell transcriptomics, computational biology, and data visualization.
- Sunshine, S. et al. (2023) ‘Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq’, Nature Communications, 14(1). Available at: http://dx.doi.org/10.1038/s41467-023-41788-4.
- Hein, M.Y. and Weissman, J.S. (2021) ‘Functional single-cell genomics of human cytomegalovirus infection’, Nature Biotechnology, 40(3), pp. 391–401. Available at: http://dx.doi.org/10.1038/s41587-021-01059-3.
- Cho, N.H. et al. (2022) ‘OpenCell: Endogenous tagging for the cartography of human cellular organization’, Science, 375(6585). Available at: http://dx.doi.org/10.1126/science.abi6983.
- Hein, M.Y. et al. (2015) ‘A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances’, Cell, 163(3), pp. 712–723. Available at: http://dx.doi.org/10.1016/j.cell.2015.09.053.
- Wiśniewski, J.R. et al. (2014) ‘A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards’, Molecular & Cellular Proteomics, 13(12), pp. 3497–3506. Available at: http://dx.doi.org/10.1074/mcp.m113.037309.