Center for Pathobiochemistry and Genetics (Institute of Medical Genetics )
Position: Research Associate (Postdoc)
ORCID: 0000-0002-2527-8012
T +43 1 40160 56558
matthew.clarke@meduniwien.ac.at
Keywords
Caenorhabditis elegans; Genes, rRNA; Neurodegenerative Diseases; Ribosomes
Research group(s)
- Schosserer Lab
Members:
Research interests
My main research interests involve understanding how chemical modifications of ribosomal RNA affects stress resistance and healthspan; especially in the context of neurodegenerative disease.
Techniques, methods & infrastructure
I mostly employ a worm model (C. elegans) to study loss of specific rRNA modifications in the context of healthspan. Some worm strains possess transgenes which express human aggregate prone proteins in worm muscle tissue. My main techniques involve RNAi knockdown of specific genes coupled with assays which measure worm motility over time. Other main assays I use include qPCR to verify RNAi knockdowns and SEM to assess the affect on cuticle integrity.
Selected publications
- Clarke, M.N. et al. (2022) ‘Adaptation to high rates of chromosomal instability and aneuploidy through multiple pathways in budding yeast’, The EMBO Journal, 42(8). Available at: https://doi.org/10.15252/embj.2022111500.
- Ravichandran, M.C. et al. (2018) ‘Genetic interactions between specific chromosome copy number alterations dictate complex aneuploidy patterns’, Genes & Development, 32(23–24), pp. 1485–1498. Available at: https://doi.org/10.1101/gad.319400.118.
- Partolina, M. et al. (2017) ‘Global histone modification fingerprinting in human cells using epigenetic reverse phase protein array’, Cell Death Discovery, 3(1). Available at: https://doi.org/10.1038/cddiscovery.2016.77.