Cell Biology; Muscle, Smooth, Vascular; RNA, Double-Stranded; RNA, Messenger; Transcriptome
We study the impact of epitranscriptomic changes and RNA-modifications on gene expression and gene egulation using transgenic mice and tissue culture cells. A key focus is currently on the interplay of RNA modifications and the biophysical properties of RNA modifications. Further, we aim to understand the regulation of the cytoskeletal protein Filamin A by RNA modification. Here we study the role of Filamin on smooth muslce contraction. Another research focus aims at deciphering the way how nucleic acids are recognized by the immune system depending on their modification state.
Techniques, methods & infrastructure
We use mice as a model to study the impact of transciptomic changes. Next generation sequencing, RNA analysis, and state-of-the-art cell biology and imaging are part of our research repertoire which we blend with standard molecular biological techniques.
A special focus lies on methods to study smooth muscle cells and their contractile behavior.
Also, using RNA Seq analysis we aim at understanding transcriptomic changes in the absence of RNA editing that may trigger an inflammatory response. This is supported by mouse genetics and cell biological model systems.
- ROPES (2021)
Source of Funding: EU, ITN
- RNA-DECO, decorating RNA for a purpose (2020)
Source of Funding: FWF (Austrian Science Fund), SFB
Coordinator of the collaborative project
- Regulation and Impact of Filamin A RNA editing (2019)
Source of Funding: FWF (Austrian Science Fund), Individual project
- Mobilis-recognition of a bimodular nuclear localization signal (2016)
Source of Funding: FWF (Austrian Science Fund), International project
- Jain, M. et al. (2022) ‘Filamin A pre-mRNA editing modulates vascularization and tumor growth’, Molecular Therapy - Nucleic Acids, 30, pp. 522–534. Available at: http://dx.doi.org/10.1016/j.omtn.2022.11.004.
- Jain, M. et al. (2022) ‘A‐to‐I RNA editing of Filamin A regulates cellular adhesion, migration and mechanical properties’, The FEBS Journal, 289(15), pp. 4580–4601. Available at: http://dx.doi.org/10.1111/febs.16391.
- Kapoor, U. et al. (2020) ‘ADAR-deficiency perturbs the global splicing landscape in mouse tissues’, Genome Research, 30(8), pp. 1107–1118. Available at: http://dx.doi.org/10.1101/gr.256933.119.
- Bajad, P. et al. (2020) ‘An internal deletion of ADAR rescued by MAVS deficiency leads to a minute phenotype’, Nucleic Acids Research, 48(6), pp. 3286–3303. Available at: http://dx.doi.org/10.1093/nar/gkaa025.
- Licht, K. et al. (2019) ‘A high resolution A-to-I editing map in the mouse identifies editing events controlled by pre-mRNA splicing’, Genome Research, 29(9), pp. 1453–1463. Available at: http://dx.doi.org/10.1101/gr.242636.118.