Endocytosis; Muscles; Neuromuscular Junction; Protein Trafficking; Proteomics; Receptor Tyrosine Kinases; Signal Transduction
- Transport and Signal Transduction of Surface Receptors
Head: Ruth Herbst
Research Area: We are interested in how receptor tyrosine kinases induce intracellular signaling cascades thereby regulating crucial cellular process including cell proliferation, differentiation and survival. Further, we are interested in trafficking processes such as endocytosis and recycling of surface proteins. We study process in the muscle and in immune cells
My work is focused on characterizing how receptor tyrosine kinases induce intracellular signaling cascades thereby regulating crucial cellular processes including cell proliferation, differentiation and function. My long-term interests center around the receptor tyrosine kinase MuSK. As postdoctoral fellow and subsequently as independent group leader, together with my laboratory, I made important contributions to the function of MuSK and to the characterization of downstream signaling events. More recently I have also become interested in protein trafficking and the interplay between signaling and protein endocytosis. In this respect, we have identified a novel guanidine nucleotide exchange factor with potential function in T cell responses during inflammatory conditions.
Techniques, methods & infrastructure
Experimental strategies include muscle cell cultures and their manipulation (retroviral-mediated gene transduction), confocal microscopy as well as in vivo imaging and analysis of transgenic animal models. Biochemistry, molecular biology and cell biology techniques complete our approaches.
- Koneczny, I. & Herbst, R., 2019. Myasthenia Gravis: Pathogenic Effects of Autoantibodies on Neuromuscular Architecture. Cells, 8(7), p.671. Available at: http://dx.doi.org/10.3390/cells8070671.
- Camurdanoglu, B.Z. et al., 2016. MuSK Kinase Activity is Modulated By A Serine Phosphorylation Site in The Kinase Loop. Scientific Reports, 6(1). Available at: http://dx.doi.org/10.1038/srep33583.
- Dürnberger, G. et al., 2014. Global Analysis of Muscle-specific Kinase Signaling by Quantitative Phosphoproteomics. Molecular & Cellular Proteomics, 13(8), pp.1993–2003. Available at: http://dx.doi.org/10.1074/mcp.M113.036087.
- Hanada, T. et al., 2013. CLP1 links tRNA metabolism to progressive motor-neuron loss. Nature, 495(7442), pp.474-480. Available at: http://dx.doi.org/10.1038/nature11923.
- Woller, B. et al., 2011. Rin-like, a novel regulator of endocytosis, acts as guanine nucleotide exchange factor for Rab5a and Rab22. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1813(6), pp.1198-1210. Available at: http://dx.doi.org/10.1016/j.bbamcr.2011.03.005.