Bacterial Outer Membrane Proteins; Blood Platelets; Hemic and Immune Systems; Platelet Activation; Puumala virus; Thrombosis
The role of platelets and platelet-pathogen interactions in inflammation and infection
We are interested in the roles of platelets and platelet-pathogen interactions in inflammation and infection. Platelets are the second most abundant circulating blood cell and fulfil essential functions in haemostasis by maintaining vascular integrity and limiting blood loss upon injury. In recent years it became increasingly apparent that platelets are not only important in haemostasis and thrombosis, but they can also fine-tune immune responses and vice versa are modulated by inflammatory or infectious conditions.
Upon activation platelets secrete a plethora of effector molecules including cytokines, chemokines and immunoglobulins that can modulate leukocyte and endothelial cell function or directly act on pathogens. Due to their high sensitivity, rapid responsiveness, and sheer number, platelets function as both sentinels and as first line of defence against invading pathogens.
We investigate the molecular crosstalk between platelet, leukocytes and bacterial and viral pathogens, the consequences of these interactions for the prothrombotic and immunomodulatory roles of platelets in acute and chronic inflammation and infection, and the impact of antiplatelet therapies in this context.
Techniques, methods & infrastructure
- Characterisation of various leukocyte subsets and activation status (mouse and human) via multi-colour flow cytometry and fluorescence microscopy
- Characterization of platelet-pathogen interactions (Puumala Hantavirus, Influenza virus, Vibrio cholerae bacteria)
- Isolation, characterization and cultivation of human and murine primary cells (platelets, leukocyte subsets, endothelial cells and hepatocytes)
- Isolation of bacterial outer membrane vesicles
- Thrombosis and haemostasis animal models (FeCl3 and mechanic injury, tail bleeding)
- Inflammation and infection animal models (peritonitis, acid- or LPS-induced acute lung injury, influenza infection, pneumococcal pneumonia)
- Transgenic mice for various platelet function deficiencies and thrombocytopenia
- Platelet function analysis (e.g. light transmission aggregometry)
- Live cell microscopy under flow conditions for recruitment and extravasation assays
- Standard biomolecular tools (PCR, qPCR, ELISA, Western Blot, cell culture)
- Viral haemorrhagic fevers in Europe: Impact of Puumala Hantavirus infection on primary haemostasis (2020)
Source of Funding: OeNB (Oesterreichische Nationalbank), Jubiläumsfonds / Anniversary Fund
- Can bacterial outer membrane vesicles drive haemorrhagic diseases? (2019)
Source of Funding: Medical Scientific Fund of the Mayor of the City of Vienna, Medizinisch-Wissenschaftlicher Fonds des Bürgermeisters der Bundeshauptstadt Wien
- Schrottmaier, W.C. et al., 2021. Adverse Outcome in COVID-19 Is Associated With an Aggravating Hypo-Responsive Platelet Phenotype. Frontiers in Cardiovascular Medicine, 8. Available at: http://dx.doi.org/10.3389/fcvm.2021.795624.
- Schrottmaier, W.C. et al., 2020. Platelets mediate serological memory to neutralize viruses in vitro and in vivo. Blood Advances, 4(16), pp.3971–3976. Available at: http://dx.doi.org/10.1182/bloodadvances.2020001786.
- Schrottmaier, W.C. et al., 2020. Platelet-leukocyte interplay during vascular disease. Atherosclerosis, 307, pp.109–120. Available at: http://dx.doi.org/10.1016/j.atherosclerosis.2020.04.018.
- Schrottmaier, W.C. et al., 2016. Platelet activation at the onset of human endotoxemia is undetectable in vivo. Platelets, 27(5), pp.479–483. Available at: http://dx.doi.org/10.3109/09537104.2015.1119814.
- Schrottmaier, W. et al., 2015. Aspirin and P2Y12 Inhibitors in platelet-mediated activation of neutrophils and monocytes. Thrombosis and Haemostasis, 114(09), pp.478–789. Available at: http://dx.doi.org/10.1160/TH14-11-0943.