Detail

Keywords

Biomarkers, Pharmacological; Epithelial-Mesenchymal Transition; Neoplasm Metastasis; Signal Transduction

Research group(s)

• Tumor Progression and Metastasis
  Members:
  Wolfgang Mikulits

Research interests

• Mechanisms of epithelial to mesenchymal transition and liver carcinoma progression
• Tumor-promoting cell signaling in liver cancer metastasis
• Biomarker of liver cancer
• Models of liver carcinoma progression
• Selective translation control during tumor progression and metastasis

Techniques, methods & infrastructure

• Molecular cloning
• Cell culture of primary cells and cell lines
• 3-dimensional culture
• Real-time qPCR
• RNA immunoprecipitation
• Flow cytometric analysis
• ELISA; Western blot; IHC; Confocal IF
• CRISPR/Cas9; RNA interfence
• Xenograft mouse models
• Syngeneic mouse models
• Invasion and metastasis assays
• Transcriptome profiling
• Proteomic profiling

Grants

• International PhD Program in Translational Oncology (2019)
  Source of Funding: FWF (Austrian Science Fund), doc.funds Programme
  Principal Investigator
• Role of Axl Receptor Shedding in Liver Cancer (2019)
  Source of Funding: FWF (Austrian Science Fund), Stande Alone Project
  Principal Investigator
• Diagnostic and Prognostic Value of Soluble Axl in Hepatocellular Carcinoma (2015)
  Source of Funding: OeNB (Oesterreichische Nationalbank), Anniversary Fund
  Principal Investigator
• Release of Soluble Axl and Gas6-Independent Axl Signaling in Hepatocellular Carcinoma (2015)
  Source of Funding: Herzfelder'sche Familienstiftung,
  Principal Investigator
• Control of Pro- and Anti-Oncogenic Stat3 Activity in Hepatocellular Carcinoma (2012)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• IT-LIVER - Strategy to Inhibit TGF-beta in Liver Disease (project partner) (2012)
  Source of Funding: EU, FP7-PEOPLE-2012-ITN
  Principal Investigator
• The Role of Axl in Hepatocellular Carcinoma Progression (2012)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator
• Cooperation of wnt beta-catenin during Hepatocellular Carcinoma Progression (2009)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• Functional analysis of Laminin B1 IRES in cancer progression (2008)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator
• Migration and Anoikis during Hepatocellular Carcinoma Progression (2008)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• RESOLVE Chronic Inflammation and Achieve Healthy Aging by Understanding Non-regenerative Repair (2008)
  Source of Funding: EU, HEALTH-F4-2008-202047
  Principal Investigator
• The Impact of the Tumor Microenvironment in Liver Carcinoma Progression (2008)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• Liver cancer stemness and metastasis (2007)
  Source of Funding: FWF (Austrian Science Fund), Stand Alone Project
  Principal Investigator
• Jak-Stat - Signalling from Basis to Disease (project part leader) (2005)
  Source of Funding: FWF (Austrian Science Fund), Special Research Programmes (SFB 28)
  Principal Investigator
• Orthotope Transplantation von Hepatozyten zur Untersuchung der Tumor-suppressiven Wirkung von c-Fos während der Lebertumorigenese (2005)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• Stats in Liver Fibrosis and Late Stage Heptocarcinogenesis (2005)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• PDGF signaling during hepatocellular tumor (2003)
  Source of Funding: OeNB (Oesterreichische Nationalbank), Anniversary Fund
  Principal Investigator
• Molecular Mechanisms Involved in Hepatocellular Epithelial to Fibroblastoid Conversion (2002)
  Source of Funding: FWF (Austrian Science Fund), Stand Alone Project
  Principal Investigator
• The Induction of Hepatocellular De-differentiation through the Cooperation of Ha-Ras and TGF-beta (2001)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator

Selected publications

1. Transforming Growth Factor-β and Axl Induce CXCL5 and Neutrophil Recruitment in Hepatocellular Carcinoma. Hepatology, 69:222-236.
2. Signaling of Axl via 14-3-3zeta Activates Autocrine Transforming Growth Factor-β Signaling in Hepatocellular Carcinoma. Hepatology, 61, 930-941.
3. p19ARF/p14ARF controls oncogenic functions of Stat3 in hepatocellular carcinoma. Hepatology, 54, 164-172.
4. PDGF essentially links TGF-β signaling to nuclear β-catenin accumulation in hepatocellular carcinoma progression. Oncogene 26, 3395-3405.
5. Hepatocytes convert to a fibroblastoid phenotype through the cooperation of TGF-beta1 and Ha-Ras: steps towards invasiveness. J. Cell Sci. 15, 1189-1202.