Detail

Keywords

Cell Migration Assays; Cytoskeleton; Microscopy; Skin

Research group(s)

• Tissue Homeostasis Group
  Head: Karin Pfisterer
  Members:
  Karin Pfisterer
  David Samardzic
  Parvaneh Balsini

Research interests

My special interest lies in regulatory signalling networks that preserve homeostasis in mammalian tissues and how this balance is disrupted during pathological processes.

Techniques, methods & infrastructure

live imaging in 2D and 3D, superresolution microscopy (PALM, STORM, SIM), FLIM-FRET, ratiometric FRET, FRAP, LLSM, primary cell isolation and in vitro differentiation, Co-IP, Ca2+ flux, lipid raft isolation, qPCR, flow cytometry, molecular cloning

Grants

• Pioneer Melanoma Cells (2021)
  Source of Funding: ARC (Austrian Research Council), Fellinger Krebsforschung
  Principal Investigator
• Allergic Skin Diseases (2020)
  Source of Funding: NÖ Forschungs- und Bildungsges.m.b.H. (NFB), DARC
  Principal Investigator
• Relocation Grant (2019)
  Source of Funding: FFG (Austrian Research Promotion Agency), Karriere-Grants
  Principal Investigator

Selected publications

1. Pfisterer, K. et al. (2021) ‘The Extracellular Matrix in Skin Inflammation and Infection’, Frontiers in Cell and Developmental Biology, 9. Available at: http://dx.doi.org/10.3389/fcell.2021.682414.
2. Pfisterer, K. et al., 2014. The Late Endosomal Transporter CD222 Directs the Spatial Distribution and Activity of Lck. The Journal of Immunology, 193(6), pp.2718–2732. Available at: http://dx.doi.org/10.4049/jimmunol.1303349.
3. Pfisterer, K. et al., 2015. CD90 + Human Dermal Stromal Cells Are Potent Inducers of FoxP3 + Regulatory T Cells. Journal of Investigative Dermatology, 135(1), pp.130–141. Available at: http://dx.doi.org/10.1038/jid.2014.309.
4. Fox-Roberts, P. et al., 2017. Local dimensionality determines imaging speed in localization microscopy. Nature Communications, 8(1). Available at: http://dx.doi.org/10.1038/ncomms13558.
5. Jayo, A. et al., 2016. Fascin Regulates Nuclear Movement and Deformation in Migrating Cells. Developmental Cell, 38(4), pp.371–383. Available at: http://dx.doi.org/10.1016/j.devcel.2016.07.021.