Hemodynamics; Neuroimaging; Ophthalmology; Tomography, Optical Coherence
My main research interests are focused on the development and application of optical imaging techniques, specifically optical coherence tomography (OCT), to study biomedical systems for preclinical or clinical research. In particular, I am interested in the role of microvascular hemodynamics in the progression of various brain and eye diseases.
Techniques, methods & infrastructure
I primarily use Optical Coherence Tomography (OCT) for brain and eye imaging in small animal (mouse and rat) models. In addition to traditional OCT methods, I have also developed a technique called Dynamic Contrast OCT (DyC-OCT) for quantitatively imaging tracer kinetics, flow, and volume.
- Merkle, C.W. & Srinivasan, V.J., 2016. Laminar microvascular transit time distribution in the mouse somatosensory cortex revealed by Dynamic Contrast Optical Coherence Tomography. NeuroImage, 125, pp.350-362. Available at: http://dx.doi.org/10.1016/j.neuroimage.2015.10.017.
- Merkle, C.W., Leahy, C. & Srinivasan, V.J., 2016. Dynamic contrast optical coherence tomography images transit time and quantifies microvascular plasma volume and flow in the retina and choriocapillaris. Biomedical Optics Express, 7(10), p.4289. Available at: http://dx.doi.org/10.1364/BOE.7.004289.
- Merkle, C.W. et al., 2018. Visible light optical coherence microscopy of the brain with isotropic femtoliter resolution in vivo. Optics Letters, 43(2), p.198. Available at: http://dx.doi.org/10.1364/OL.43.000198.
- Chong, S.P. et al., 2015. Quantitative microvascular hemoglobin mapping using visible light spectroscopic Optical Coherence Tomography. Biomedical Optics Express, 6(4), p.1429. Available at: http://dx.doi.org/10.1364/BOE.6.001429.
- Chong, S.P. et al., 2015. Noninvasive, in vivo imaging of subcortical mouse brain regions with 17��μm optical coherence tomography. Optics Letters, 40(21), p.4911. Available at: http://dx.doi.org/10.1364/OL.40.004911.